Rapid detection of feline morbillivirus by a reverse transcription loop-mediated isothermal amplification
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چکیده
منابع مشابه
Rapid detection of feline morbillivirus by a reverse transcription loop-mediated isothermal amplification
Feline morbillivirus (FmoPV) is an emerging virus in domestic cats and considered to be one of the causes of chronic renal failure in cats. In this study, we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of FmoPV. The results indicated that the detection limit of the assay was 10 50% tissue culture infective dose (TCID50)/ml in the ...
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The fragment of the membrane protein M gene with high conservation and specificity of porcine reproductive and respiratory syndrome virus (PRRSV) was chosen to be the target region, according to which six special primers were designed successfully. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) was applied to detect the PRRSV by incubation at 65° for only 45 min with the...
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BACKGROUND Bovine rotavirus (BRV) infection is common in young calves. This viral infection causes acute diarrhea leading to death. Rapid identification of infected calves is essential to control BRV successfully. Therefore development of simple, highly specific, and sensitive detection method for BRV is needed. RESULTS A reverse transcription loop-mediated isothermal amplification (RT-LAMP) ...
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ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 2016
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.15-0239